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Development and research of chlorophyll meter

Nov 21, 2018

Chlorophyll in plant leaves plays an important role in photosynthesis, and its content is an important indicator for evaluating plant physiological status. By measuring the concentration of chlorophyll, it is possible to accurately determine the growth of plants. To measure chlorophyll using traditional spectrophotometry, the sample is extracted with acetone and the sample is measured using a spectrophotometer. Although the traditional spectroscopic method has high measurement accuracy, this method of damage damages plant leaves, takes a long time, is not convenient for continuous measurement, and cannot meet the requirements of rapid and non-destructive testing in the field. Therefore, in view of the current lack of high-precision, low-cost field chlorophyll rapid detection equipment for field crops in China, a portable chlorophyll detection device based on single-chip microcomputer is researched and developed. The chlorophyll meter was developed in such a state.

There are two main methods for measuring the leaf chlorophyll content of living plants: reflection and transmission. The reflection method can be used to determine the condition of a vegetation group in a field, and can also be used to determine a single leaf. The instrument used in the reflection method is complicated, and the reflection of the light on the blade is related to the surface condition of the blade, and also related to the distribution of chlorophyll in the blade. Therefore, the error is relatively large, and the chlorophyll determination in the sea area is mostly applied. When measuring the chlorophyll content in leaves by transmission method, it is necessary to consider the influence of other pigments in the blade, the shape of the blade and the position and time of the measurement of the blade on the measurement. The dual wavelength method is generally used to eliminate this effect.

The chlorophyll meter is designed to facilitate data storage and is designed with an SD card module. At the same time, in order to reflect the geographical information of the measured blade in real time, the GPS module is designed as a peripheral device, and the RS232 module is designed as the communication port. The instrument overcomes the shortage of data storage in other similar products, and adds GPS function, which realizes the measurement of the location information of the measurement location, and has the functions of saving time, date, ambient temperature and humidity and other information. Compared with the Japanese SPAD-502 instrument, the correctness and achievability of the instrument for photoelectric nondestructive detection of chlorophyll content were proved. The instrument is easy to carry and suitable for field testing, which is easy to promote in the market.

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