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Analysis of relationship between chlorophyll value and SPAD value by portable chlorophyll meter

Feb 16, 2019

Plant photosynthesis is inseparable from chlorophyll. The chlorophyll content in leaves directly determines the efficiency of plant photosynthesis, and can be used as the main reference for reflecting physiological changes such as plant nutrient level, stress resistance and aging. At present, the determination of chlorophyll content can be mainly divided into two ways, the spectrophotometer method and the chlorophyll analyzer method. The former has great damage to the blade, and the operation is complicated, which is time-consuming and laborious; the latter non-destructive measurement of the leaf chlorophyll, saving time and labor.


The portable chlorophyll meter mainly uses the SPAD value to understand the chlorophyll content. The SPAD value is the so-called chlorophyll value. In recent years, many studies have shown that most plant leaf values have a significant positive correlation with their chlorophyll content, but there are some differences in the models of different plants using SPAD values to predict leaf chlorophyll content. Therefore, this experiment uses alpine rhododendron as a material to compare the different methods for determining the chlorophyll content of the leaves, and analyzes the relationship between the measured values of chlorophyll and SPAD values, which provides a reference for the application of portable chlorophyll analyzer to predict the chlorophyll content in vivo.


Through the portable chlorophyll analyzer, it was found that the SPAD value and the chlorophyll content of the rhododendron were positively correlated. As the SPAD value increased, the chlorophyll content increased accordingly. In summary, the best correlation function between chlorophyll a, chlorophyll b and SPAD values is a power function model. This may be because the portable chlorophyll meter has a working light source of 660 nm, which is close to the maximum absorption peak wavelength of chlorophyll a and is far from the maximum absorption peak wavelength of chlorophyll b. The measuring instrument measures the chlorophyll content of rapeseed, so that in the process of processing and producing oil, people can reasonably control the chlorophyll content in the oil, thereby effectively improving the quality of the oil and maximizing the benefit of the rape.


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